Abstract: Gulong Sag in Songliao Basin has good conditions for shale oil enrichment and promising exploration and development potential,and large-scale development tests have been conducted. At present, there is a lack of deep research on the subdivided sequence stratigraphy of Qingshankou Formation, which affects the comprehensive geological study, sweet spot prediction, and exploration deployment of Gulong shale oil. Guided by the theory of seismic sequence stratigraphy and based on the characteristics of relatively stable lateral sedimentation of shale type shale oil, No. 5 test zone of Gulong shale oil is studied as an example, and seismic processing, interpretation (i.e., anisotropic diffusion filtering, sequence recognition and picking), and geology are integrated to identify geological horizons by seismic waveforms,forming a sequence framework construction technology by using seismic waveforms, which has achieved rapid transformation of seismic waveforms into geological sedimentary sequences. In the study area, a total of one secondary, one tertiary, and eight fourth-order sequence boundaries are identified in Qingshankou Formation, and nine thin layers (Q₁-Q₉) are subdivided. The framework characteristics of all thin layers have been analyzed, laying the foundation for shale lithofacies identification and fine prediction of sweet spots. By analyzing four shale evaluation parameters, including TOC, sedimentary structure, mineral composition, and lamina density, a classification standard for shale type lithofacies of Gulong shale has been established based on the subdivided stratigraphic framework, and 10 sub lithofacies have been identified.For Q₁-Q₄ thin layers, three types of sub lithofacies are subdivided, and their plane distribution characteristics are characterized. Based on the above research and the prediction results by using six shale oil sweet spot evaluation parameters such as oil content, brittleness, and physical properties, 11 wells have optimally been deployed in No. 5 test zone, and an average oil rate of more than 10 t/d has been achieved, effectively supporting the beneficial exploration and development of Gulong shale oil in Songliao Basin.