Abstract: Comprehensive analysis of pre-salt stratigraphic sequence, thermal history and trap evolution of Lula Oilfield (formerly known as Tupi Oilfield) in Santos Basin, Brazil, shows that the source rock of the oilfield is lacustrine shale of middle-upper rift sequence; its reservoir rock is carbonate of sag sequence; and its trap type is a lithologic-structural combination trap controlled by horsts formatting during Neocomian- Barremian rift stage, that trap evolution of Lula Oilfield experienced six stags, i.e. the early rift, middle rift and late rift stages of Neocomian- Barremian, the sag stage of early Aptian, evaporite stage of late Aptian and the pool formation stag from Albian to now, and that its accumulation feature presents subsalt deeper reservoir, lately gathering and medium mature of source rock. It is concluded that the very thick salt layer (~2000m) effect in distribution and high heat conductivity greatly delayed thermal evolution degree of pre-salt source rocks, and expanded the hydrocarbon exploration depth for the pre-salt section. In addition, the oil-gas accumulation zone of horst-stratum becomes a new exploratory frontier for the whole pre-salt sections, not only in Santos, but also throughout the eastern Brazilian offshore basins