Abstract: The paper makes statistical analysis of 97 wells drilled in the eastern part and hinterland of Junggar Basin and integrated assessment on hydrocarbon source rock in terms of Carboniferous hydrocarbon source rock distribution, organic matter type, abundance and thermal evolution degree. The paper makes study of Carboniferous hydrocarbon generation capacity. Carboniferous source rock is extensively distributed in the eastern part and hinterland of Junggar Basin, mainly tuff and sedimentary tuff. Lower Carboniferous hydrocarbon source rock is distributed mainly in the Dishuiquan and Wucaiwan area. It is promising source rock of sapropelic-humic type under the reduction environment. Upper Carboniferous source rock is distributed mainly in the regions of Dajing, Zhangbei Shixi, Xiayean and Wucaiwan in the eastern part of the basin. It is the relatively desirable source rock of the mature sapropelic-humic type under the weak reduction to reduction environment. The Carboniferous self-sourcing and self-stored oil and gas is also found in the recent years, indicating a promising prospect for exploration of Carboniferous.